In this session, we will continue to look at full spectrum flow cytometry and look at considerations for fluorescence panel design. What do we have to think about to design a successful panel and how can we combine fluorochromes that might be difficult on a conventional flow cytometer? We will also look again at spectral unmixing and present some troubleshooting tips. The session will be a mixture of theory and practice using a 5 laser Cytek Aurora. This webinar is a collaboration between Derek Davies (The Francis Crick Institute, London, UK) and Kathy Daniels and Rui Gardner (Memorial Sloan Kettering Cancer Center, New York, USA).